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Protocols

Protocols for the Detection of Renibacterium salmoninarum in salmon

Photo of BKD in a juvenile salmon

Kidney lesions in a juvenile Chinook salmon (Oncorhynchus tshawytscha) affected by bacterial kidney disease (BKD). Credit: Caroline Bentley, PhD., University of Central Oklahoma.

Renibacterium salmoninarum is a fastidious, gram-positive bacterium and the causative agent of bacterial kidney disease (BKD) in wild and cultured salmonid fishes. A number of diagnostic tests exist for the direct and indirect detection of R. salmoninarum. These tests have different analytical properties and different abilities to quantify infection levels, detect remote infections and distinguish between live and dead bacteria. This website links to standard operating procedures (SOP) for the major R. salmoninarum diagnostic tests.

For more information, contact Dr. Maureen Purcell or Dr. Diane Elliott at the Western Fisheries Research Center.








Culture of Renibacterium salmoninarum Quantitative Culture for R. salmoninarum - (2011) (Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid)
Enzyme linked immunosorbent assay (ELISA) Enzyme linked immunosorbent assay (ELISA) for R. salmoninarum – (2012) (Double Polyclonal Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of Renibacterium salmoninarum)
Direct fluorescent antibody test (DFAT) Direct fluorescent antibody test (DFAT) – (2011) (Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues)
Membrane filtration fluorescent antibody test (MF-FAT) Membrane filtration fluorescent antibody test (MF-FAT) – (2012)(Membrane-Filtration Fluorescent Antibody Test (MF-FAT) for the detection of Renibacterium salmoninarum)
DNA extraction from gram positive bacteria DNA extraction for gram positive bacteria – (2011)(DNA Extraction for PCR-based detection of Renibacterium salmoninarum)
Nested PCR (nPCR) test Nested PCR (nPCR) test – (2011)(Nested Polymerace Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum)
Quantitative PCR (qPCR) test Quantitative PCR (qPCR) test – (Quantitative Polymerace Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum)




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